Journal: Microbial Pathogenesis

Article Title: Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus

doi: 10.1016/j.micpath.2020.104560

Figure Lengend Snippet: Representative results showing distributions of (A) CD3 + CD4 + and (B) CD3 + CD8 + T-lymphocytes from IB DNA vaccinated chickens (group E) as determined by flow cytometry.

Article Snippet: A single cell suspension was prepared at a concentration of 1 × 10 7 cells/ml and about 100 μl of cell suspensions (1 × 10 6 cells) were incubated for 2 h at 4 °C with mouse anti-chicken CD3-FITC (Southern Biotech, USA), mouse anti-chicken CD4-R-phycoerythrin (R-PE) (Southern Biotech, USA), and mouse anti-chicken CD8 cy5 (Southern Biotech, USA).

Techniques: Flow Cytometry

Journal: Microbial Pathogenesis

Article Title: Development and immunogenic potentials of chitosan-saponin encapsulated DNA vaccine against avian infectious bronchitis coronavirus

doi: 10.1016/j.micpath.2020.104560

Figure Lengend Snippet: Percentage CD3 + CD4 + and CD3 + CD8 + T-lymphocytes in vaccinated and control chicken groups 2 weeks after booster vaccination with IB - DNA vaccines. Vaccination regimen and control includes: (A) PBS; (B) pBudCE4.1 (naked DNA); (C) pBudCR88-S1; (D) pBudM41-S1; (E) pBudCR88S1/M41S1+Nano; (F) pBudCR88S1/M41S1- Nano. Evaluations of Clinical signs and viral shedding after challenge.

Article Snippet: A single cell suspension was prepared at a concentration of 1 × 10 7 cells/ml and about 100 μl of cell suspensions (1 × 10 6 cells) were incubated for 2 h at 4 °C with mouse anti-chicken CD3-FITC (Southern Biotech, USA), mouse anti-chicken CD4-R-phycoerythrin (R-PE) (Southern Biotech, USA), and mouse anti-chicken CD8 cy5 (Southern Biotech, USA).

Techniques: Control, Vaccines

Journal: Frontiers in immunology

Article Title: Development and characterization of a CRISPR/Cas9-mediated RAG1 knockout chicken model lacking mature B and T cells.

doi: 10.3389/fimmu.2022.892476

Figure Lengend Snippet: FIGURE 6 Immune cell populations and quantification of secreted immunoglobulins in RAG1 knockout chickens. Representative flow cytometry analysis of B and T cell subpopulations in the bursa (A), and thymus (B) of 3-week-old chickens. Statistical analysis was performed on Bu-1 and IgM positive cells in the bursa as well as CD4+ and CD8+ single-positive (SP) T cells, and the CD4+CD8+ double-positive (DP) T cell lymphocyte subpopulation in the thymus. Serum IgM, IgY, and IgA levels in 1-week (C) and 3-week (D) -old WT, RAG1+/-, and RAG1-/- chickens, as determined by ELISA. Each dot represents an individual chicken. The significance of differences among groups was assessed by one-way ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001, ns, no significance.

Article Snippet: Cells (1 × 106) were stained for 40 min on ice using the following antibodies: mouse anti-chicken Bu-1 FITC (8395-02), mouse anti-chicken IgM BIOT (8310-08), mouse anti-chicken CD45 SPRD (8270-13), mouse antichicken CD3 FITC (8200-02), mouse anti-chicken CD4 Alexa Fluor 647 (8201-31), mouse anti-chicken CD8a BIOT (8405- 08), mouse IgG1-FITC (0102-02), mouse IgM-SPRD (0101-13), mouse IgG2b-BIOT (0104-08) and mouse IgG1-Alexa Fluor 647 (0102-31) were purchased from Southern Biotech (Birmingham, AL, USA).

Techniques: Knock-Out, Cytometry, Enzyme-linked Immunosorbent Assay